Role and uses Immortalised cell line

1 role , uses

1.1 limitations

1.1.1 changes nonimmortal origins
1.1.2 contamination other cells

role , uses

immortalised cell lines used simple model more complex biological systems, example analysis of biochemistry , cell biology of mammalian (including human) cells. main advantage of using immortal cell line research immortality; cells can grown indefinitely in culture. simplifies analysis of biology of cells may otherwise have limited lifetime.

immortalised cell lines can cloned giving rise clonal population can, in turn, propagated indefinitely. allows analysis repeated many times on genetically identical cells desirable repeatable scientific experiments. alternative, performing analysis on primary cells multiple tissue donors, not have advantage.

immortalised cell lines find use in biotechnology cost-effective way of growing cells similar found in multicellular organism in vitro. cells used wide variety of purposes, testing toxicity of compounds or drugs production of eukaryotic proteins.

limitations
changes nonimmortal origins

while immortalised cell lines originate well-known tissue type, have undergone significant mutations become immortal. can alter biology of cell , must taken consideration in analysis. further, cell lines can change genetically on multiple passages, leading phenotypic differences among isolates , potentially different experimental results depending on when , strain isolate experiment conducted.

contamination other cells

many cell lines used biomedical research have been contaminated , overgrown other, more aggressive cells. example, supposed thyroid lines melanoma cells, supposed prostate tissue bladder cancer, , supposed normal uterine cultures breast cancer.